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Abstract
Long-term post-mitotic culture of human fibroblasts recapitulates and accelerates features of in vivo aging.
- In vitro aging of fibroblasts shows key characteristics similar to aging in living organisms.
- Directly converting aged fibroblasts into neurons maintains their biological age, allowing for modeling of neuronal aging.
- A time-dependent increase in age-heterogeneity was observed in fibroblasts, aligning with known patterns of aging variability.
- Metformin was found to broadly reduce markers of aging at the molecular level, while Rapamycin had limited effects.
- The findings support the use of this platform to explore and predict the effectiveness of anti-aging treatments.
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