Magnesium Oxide Nanoparticle Coordinated Phosphate-Functionalized Chitosan Injectable Hydrogel for Osteogenesis and Angiogenesis in Bone Regeneration

Feb 4, 2022ACS applied materials & interfaces

Injectable Gel with Magnesium Nanoparticles and Phosphate-Modified Chitosan to Support Bone and Blood Vessel Growth

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Regenerative Health on OpenScience ↗PubMed ↗DOI ↗

Abstract

The CSMP-MgO injectable hydrogel achieved a maximum compressive strength of 195.0 kPa in compression tests.

Incorporation of MgO nanoparticles into a chitosan-based hydrogel improved its mechanical properties, preventing brittle failure at strains above 85%.
The CSMP-MgO hydrogel exhibited a porous structure with pore diameters ranging from 50 to 100 μm, allowing for potential nutrient exchange.
A sustained and stable release of magnesium was observed in the CSMP-MgO hydrogels, which may support osteogenic activity.
In vitro studies showed that CSMP-MgO promoted mineralization and enhanced osteogenic differentiation of MC3T3-E1 cells.
CSMP-MgO (5) hydrogel demonstrated an ability to stimulate new bone formation in critical-sized calvarial defects in rats.

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Natural polysaccharide (NPH)-based injectable hydrogels have shown great potential for critical-sized bone defect repair. However, their osteogenic, angiogenic, and mechanical properties are insufficient. Here, MgO nanoparticles (NPs) were incorporated into a newly synthesized water-soluble phosphocreatine-functionalized chitosan (CSMP) water solution to form an injectable hydrogel (CSMP-MgO) via supramolecular combination between phosphate groups in CSMP and magnesium in MgO NPs to circumvent these drawbacks of chitosan-based injectable hydrogels. Water-soluble chitosan deviate CSMP was first synthesized by grafting methacrylic anhydride and phosphocreatine into a chitosan chain in a one-step lyophilization process. The phosphocreatine in this hydrogel not only provides sites to combine with MgO NPs to form supramolecular binding but also serves as the reservoir to control Mgrelease. As a result, the lyophilized CSMP-MgO hydrogels presented a porous structure with some small holes in the pore wall, and the pore diameters ranged from 50 to 100 μm. The CSMP-MgO injectable hydrogels were restricted from swelling in DI water (lowest swelling ratio was 16.0 ± 1.1 g/g) and presented no brittle failure during compression even at a strain above 85% (maximum compressive strength was 195.0 kPa) versus the control groups (28.0 and 41.3 kPa for CSMP and CSMP-MgO (0.5) hydrogels), with regulated Mgrelease in a stable and sustained manner. The CSMP-MgO injectable hydrogels promoted in vitro calcium phosphate (hydroxyapatite (HA) and tetracalcium phosphate (TTCP)) deposition in supersaturated calcium phosphate solution and presented no cytotoxicity to MC3T3-E1 cells; the CSMP-MgO hydrogel promoted MC3T3-E1 cell osteogenic differentiation with upregulation of BSP, OPN, and Osterix osteogenic gene expression and mineralization and HUVEC tube formation. Among them, CSMP-MgO (5) presented most of these properties. Moreover, this hydrogel (CSMP-MgO (5)) showed an excellent ability to promote new bone formation in critical-sized calvarial defects in rats. Thus, the CSMP-MgO injectable hydrogel shows great promise for bone regeneration. 2+ 2+

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Magnesium Oxide Nanoparticle Coordinated Phosphate-Functionalized Chitosan Injectable Hydrogel for Osteogenesis and Angiogenesis in Bone Regeneration

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