The Photopigment Melanopsin Is Exclusively Present in Pituitary Adenylate Cyclase-Activating Polypeptide-Containing Retinal Ganglion Cells of the Retinohypothalamic Tract

Jan 5, 2002The Journal of neuroscience : the official journal of the Society for Neuroscience

The light-sensitive pigment melanopsin is found only in special retinal cells that send signals to the brain's daily rhythm system

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Abstract

Melanopsin is found exclusively in pituitary adenylate cyclase-activating polypeptide (PACAP)-containing retinal ganglion cells.

  • Melanopsin and PACAP are localized in the same retinal ganglion cells, suggesting a specific role for melanopsin in circadian signaling.
  • The study utilized cloning of rat melanopsin photopigment cDNA and development of a specific melanopsin antibody.
  • In situ hybridization and immunohistochemistry techniques revealed that melanopsin is present on the surface of somas and dendrites of PACAP-containing cells.
  • Colocalization studies indicate that melanopsin may function as a circadian photopigment associated with the retinohypothalamic tract.
  • Findings are supported by published action spectra analyses and observations in retinally degenerated mutant mice.

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