Messenger RNA delivery into Sertoli cells restores fertility to congenitally infertile male mice

Mar 6, 2026Stem cell reports

Delivering messenger RNA to support cells restores fertility in infertile male mice

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Abstract

mRNA delivery into mouse testes restored fertility in a genetic model of infertility.

  • Injected mRNA was specifically expressed in Sertoli cells.
  • An innate immune response was triggered, but spermatogenesis resumed without major side effects.
  • Delivery of naked Cldn11 mRNA in mice with meiotic defects allowed progression from spermatocytes to spermatids.
  • Fertile offspring with normal imprinting were produced via microinsemination.
  • This approach may provide a safer alternative for treating male infertility by targeting testicular somatic cells.

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