Metabolic Biomarkers for Lifespan Extension and Cellular Stress in Saccharomyces cerevisiae during Calorie Restriction and Quercetin Treatment

Nov 3, 2025Preventive nutrition and food science

Metabolic markers linked to longer life and stress in yeast during calorie restriction and quercetin treatment

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Abstract

Aging in yeast cells is associated with increased intracellular levels and altered metabolite profiles.

The study focused on how aging affects intracellular metabolites in normal yeast cells.
and quercetin treatment significantly increased intracellular levels, which are linked to mitochondrial function.
Trehalose levels rose in response to aging, calorie restriction, and dimethyl sulfoxide treatment, suggesting a protective response to stress.
Quercetin treatment mitigated the metabolic stress induced by dimethyl sulfoxide.
Intracellular trehalose and proline may serve as valuable biomarkers for cellular stress and longevity in yeast.

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As life expectancy increases, age-related diseases pose significant challenges in modern medicine. The molecular mechanisms of aging have been widely explored in mice, nematodes, human cells, and yeast. The budding yeasthas become the most widely employed eukaryotic model due to its short lifespan and well-characterized genetic and molecular profiles. However, research into the metabolite perturbations associated with lifespan extension has tended to focus on mutant or engineered yeast cells, and information regarding these processes in normal yeast cells remains scant. In this study, therefore, we investigated how aging affects the intracellular metabolites ofduring its growth and how these changes relate to lifespan extension induced by (CR) and quercetin treatment using aH nuclear magnetic resonance (NMR)-based metabolomic approach. The results revealed clear relationships between intracellular metabolites and aging, CR, and quercetin treatment in yeast cells. The intracellular levels were found to increase with aging, CR, and dimethyl sulfoxide (DMSO) treatment, indicating that yeast cells activate protective responses against cellular stress. Meanwhile, quercetin treatment was able to clear the metabolic stress caused by DMSO treatment. The treatment of both CR and quercetin significantly increased the intracellular levels, which are known to regulate mitochondrial function and decline with age. The findings of this study suggest that CR and quercetin promote the longevity ofthrough a shared metabolic pathway and highlight intracellular trehalose and proline as potentially valuable biomarkers of cellular stress and longevity in yeast cells. Saccharomyces cerevisiae S. cerevisiae S. cerevisiae1

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Metabolic Biomarkers for Lifespan Extension and Cellular Stress in Saccharomyces cerevisiae during Calorie Restriction and Quercetin Treatment

Abstract

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