Molecular characterization of a cDNA encoding red pigment-concentrating hormone in black tiger shrimp Penaeus monodon: Implication of its function in molt and osmoregulation

Jun 18, 2014Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

Red pigment-concentrating hormone in black tiger shrimp and its possible role in molting and salt balance

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Abstract

A 770 bp full-length cDNA of red pigment-concentrating hormone (PmRPCH) was identified in the black tiger shrimp, Penaeus monodon.

PmRPCH mRNA expression was highest in eyestalks and lower in other nervous tissues such as the brain and abdominal nerve cord.
Expression of PmRPCH was stimulated within 12 hours after hypersalinity changes, indicating a possible role in osmoregulation.
During the molting cycle, PmRPCH expression peaked in the late pre-molt and post-molt stages after a gradual increase from the early pre-molt stage.
Injection of 100 pmol of RPCH peptide enhanced gill Na(+)/K(+) ATPase activity within 36-48 hours.
PmRPCH did not shorten the duration of the molting cycle but may influence hemolymph osmolality and ion transport during molting.

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Red pigment-concentrating hormone (RPCH) is a member of the AKH/RPCH peptide family present mainly in crustaceans and insects. Insect AKH is responsible for metabolic functions whereas RPCH plays a major role in the aggregation of red chromatophores in crustaceans. In this study, a full-length cDNA of RPCH of the black tiger shrimp, Penaeus monodon (PmRPCH) was cloned by Rapid Amplification of cDNA Ends strategies from the eyestalk RNA. A 770 bp full-length PmRPCH cDNA harbored 279 bp of an open reading frame encoding a signal peptide of 21 amino acid residues, an 8 amino acid mature RPCH peptide, followed by 61 amino acid residues of a RPCH precursor-related peptide. The highest levels of PmRPCH mRNA expression were detected in eyestalks while lower expression was found in other nervous tissues i.e. brain, thoracic ganglia and abdominal nerve cord. Expression of PmRPCH was transiently stimulated upon hypersalinity change within 12 h suggesting its osmoregulatory function. During the molting cycle, PmRPCH in the eyestalk was expressed at the lowest level in the early pre-molt stage (D0), then gradually increased over the pre-molt period and reached the highest level in the late pre-molt (D4) and post-molt (AB) stages. RPCH peptide at a dose of 100 pmol also increased gill Na(+)/K(+) ATPase activity in 36-48 h after injection. However, PmRPCH did not accelerate the duration of molting cycle. Our results provide the first evidence on the potential function of PmRPCH in molting, probably by mediating hemolymph osmolality and ion transport enzymes during the late pre-molt stage.

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Molecular characterization of a cDNA encoding red pigment-concentrating hormone in black tiger shrimp Penaeus monodon: Implication of its function in molt and osmoregulation

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