A mouse circadian proteome atlas

🥇 Top 1% JournalJan 10, 2026Molecular cell

Daily protein changes in mice over the 24-hour cycle

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Circadian Biology on OpenScience ↗PubMed ↗DOI ↗

Abstract

A comprehensive mouse circadian proteome atlas was generated by analyzing 32 tissues and revealing spatiotemporal profiles of about 19,000 proteins.

Next-generation DNA sequencing and mass spectrometry techniques were utilized to investigate protein expression related to the circadian clock.
The study included the analysis of 584 samples, encompassing various developmental stages.
Circadian changes in both the quantity and quality of proteins were observed in the liver.
Global changes were noted in hPER2-S662G mutant mice, a genetic model associated with human familial advanced sleep phase.
This atlas serves as a resource for understanding the timing and location of protein expression and function in relation to circadian rhythms.

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The circadian clock drives daily rhythms of gene expression and physiology. Advances in next-generation DNA sequencing have provided extensive insights into RNA expression, but more functional information at the protein level with sufficient depth has been limited by technical challenges. In this study, we generated a comprehensive mouse circadian proteome atlas (https://chronoproteinology.org/circadian_atlas) by analyzing 32 tissues, including the suprachiasmatic nucleus (SCN), using the next-generation mass spectrometer Orbitrap Astral. Data-independent acquisition of 584 samples, including developmental samples, revealed the spatiotemporal profiles of about 19,000 proteins. Proteome and phospho-proteome analyses of whole-cell and nuclear proteins in the liver revealed circadian changes in protein quantity and quality, as well as global changes in hPER2-S662G mutant mice, a genetic model of human familial advanced sleep phase (FASP). This multi-tissue circadian proteome atlas provides a fundamental resource for understanding when, where, and which proteins are expressed and function.

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