Biotechnology progressJan 30, 2026

Improving mRNA vaccine production by optimizing lab-made RNA for higher amount and quality

mRNA Technology Weekly Brief ↗PubMed ↗DOI ↗

Updated Feb 1, 2026

Abstract

Total nucleoside-5'-triphosphates (NTPs) input and magnesium concentration significantly influence the yield and purity of RNA produced by in vitro transcription.

High RNA yield and integrity are associated with specific NTP input levels and magnesium concentrations.
Low concentrations of double-stranded RNA (dsRNA) were achieved alongside high-quality RNA constructs.
Different RNA sequences and lengths can be effectively produced using the proposed IVT design.
The findings may help in creating a more cost-effective and efficient platform for RNA manufacturing.

Simplified

In vitro transcription (IVT) is a powerful method to generate RNA which not only facilitates RNA research but also plays a key role in the development and manufacture of RNA-based vaccines. mRNA is produced via the IVT process with a DNA template that contains information for the target antigen. However, as many disease-causing viruses mutate quickly and the cost of raw materials is high for the IVT reaction, there is a need for a system to develop a cost-effective and efficient IVT process platform. In this paper, we showed how total nucleoside-5'-triphosphates (NTPs) input, Mgconcentration and NTP preparation methods can influence IVT reaction yield and purity level of the final RNA constructs of different lengths and sequences. We propose an IVT design that will result in high RNA yield, high RNA integrity and low double-stranded RNA (dsRNA) concentrations for multiple RNA sequences. The approach presented here could significantly contribute to the development of a cost-effective, easy-to-adopt IVT process platform for RNA manufacturing. 2+

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Improving mRNA vaccine production by optimizing lab-made RNA for higher amount and quality

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