Detailed Analysis of Single Suspended Nanoparticles Using Advanced Fluorescence and Scattering Microscopy in Controlled Microfluidic Environments

Detailed characterization of suspended mRNA-lipid nanoparticles (LNPs) reveals their size, mass, and payload characteristics.

• The method enables simultaneous measurement of size, mass, refractive index, and nucleic acid payload of individual LNPs.
• Accuracy and precision were validated using nanosized beads.
• Different solvent pH conditions were investigated for their effects on the size, payload, and water content of LNPs.
• The approach combines conventional microscopy with advanced confinement techniques, potentially enhancing nanoparticle analysis.

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