Nano-Biomimetic Fibronectin/Lysostaphin-Co-Loaded Silk Fibroin Dressing Accelerates Full-Thickness Wound Healing via ECM-Mimicking Microarchitecture and Dual-Function Modulation

Jun 18, 2025International journal of nanomedicine

Silk Fibroin Wound Dressing with Fibronectin and Lysostaphin Speeds Healing by Mimicking Natural Tissue Structure and Providing Two Types of Support

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Regenerative Health on OpenScience ↗PubMed ↗DOI ↗OA ↗

Abstract

Fn-rLys-Col/SF-S healed skin defects one week earlier than the control group.

The biomimetic dressing demonstrated good water vapor balance and antibacterial properties.
In vitro tests indicated it is non-toxic to cells and promotes cell proliferation and migration.
The newly formed skin structure at 21 days closely resembled normal skin.
Immunohistochemistry and qRT-PCR results showed reduced inflammation and enhanced collagen deposition.
The dressing may promote angiogenesis and regulate the degree of fibrosis.

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PURPOSE: In cases of large-area skin defects, the absence of can lead to difficulties in fibroblast migration, thereby hindering wound healing. This study aimed to address the challenges in treating skin defects by developing a biomimetic nano-dressing that both has antibacterial properties and promotes healing by mimicking the extracellular matrix.

PATIENTS AND METHODS: The electrospun silk protein nanofibers were ultrasonically fragmented into staple fibers. These were then coated and modified by adding a collagen (Col) solution loaded with recombinant lysostaphin (rLys) and fibronectin (Fn), ultimately constructing a biomimetic nanosponge (Fn-rLys-Col/SF-S).

RESULTS: In vitro studies have shown that Fn-rLys-Col/SF-S possesses good water vapor balance and antibacterial properties, is non-toxic to cells, and can promote cell proliferation and migration. In vivo experimental results indicated that Fn-rLys-Col/SF-S healed a week earlier than the control group, with the structure of the newly formed skin resembling normal skin at 21 days. Further immunohistochemistry and qRT-PCR results demonstrated that Fn-rLys-Col/SF-S effectively promotes the healing of skin defect wounds by reducing inflammation, promoting angiogenesis, enhancing collagen deposition, and regulating the degree of fibrosis.

CONCLUSION: In conclusion, the Fn-rLys-Col/SF-S biomimetic sponge dressing can promote the repair of skin defects by mimicking the extracellular matrix, providing a potential therapeutic strategy for clinical wound treatment.

Key numbers

7 days

Healing Time Reduction

Fn-rLys-Col/SF-S dressing vs. control treatments

77.8±0.31%

Rate

Against MRSA

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Nano-Biomimetic Fibronectin/Lysostaphin-Co-Loaded Silk Fibroin Dressing Accelerates Full-Thickness Wound Healing via ECM-Mimicking Microarchitecture and Dual-Function Modulation

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