Natural and experimental infection of white spot syndrome virus (WSSV) in benthic larvae of mud crab Scylla serrata

Apr 27, 2000Diseases of aquatic organisms

Natural and experimental infection of white spot syndrome virus in bottom-dwelling larvae of mud crab Scylla serrata

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Abstract

WSSV was detected in around 60% of the mud crab larvae sampled from Taiwan's coastal waters.

The prevalence of WSSV remained fairly constant when larvae were maintained in a laboratory setting.
Cumulative mortality in WSSV-challenged larvae reached 43% after 15 days, compared to 20% in the control group.
PCR testing confirmed the presence of WSSV in most moribund and surviving specimens, suggesting a link to mortality.
Histological examination indicated that WSSV affects crab larvae tissue similarly to shrimp.

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White spot syndrome virus (WSSV), the causative agent of white spot syndrome in shrimp, has a wide host range which extends to crabs, copepods and other arthropods. In this study, benthic larvae of the mud crab Scylla serrata were captured from Taiwan's coastal waters and screened for the presence of WSSV by polymerase chain reaction (PCR) and in situ hybridization. WSSV was detected in around 60% of the larvae, and this prevalence rate remained fairly constant when the captured larvae were subsequently maintained in an aerated system in the laboratory. WSSV-free larvae obtained from a hatchery were challenged by immersion in a WSSV inoculum. Fifteen days after challenge, cumulative mortality in the experimental group reached 43% compared to 20% in the control group. PCR detection of WSSV in both moribund and surviving specimens clearly implicated the virus as the cause of death in most cases. Histological and in situ hybridization data confirmed that WSSV tissue tropism in Scylla serrata crab larvae is similar to that found in shrimp.

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