Journal of enzyme inhibition and medicinal chemistryNov 12, 2025

New Pyrazole Compounds and Their Potential to Protect Nerves

Updated Jan 1, 2026

Abstract

The most potent compound demonstrated an IC50 value of 9.562 μM for suppressing IL-6 expression.

27 novel pyrazole derivatives were designed and synthesized for evaluation.
The anti-inflammatory activity was assessed in lipopolysaccharide-stimulated BV2 microglial cells.
Key pro-inflammatory cytokines, including tumour necrosis factor-α, interleukin-1β, and interleukin-6, were analyzed via mRNA expression levels.
One compound showed superior inhibitory potency against IL-6 compared to dexamethasone and Celecoxib.
No significant cytotoxicity was observed for the most potent compound.

Simplified

Spinal cord injuries (SCIs) cause irreversible damage and lasting neurological impairments. Current treatments are limited to surgical and pharmaceutical interventions, underscoring the need for novel agents. In this study, 27 novel pyrazole derivatives were designed, synthesised. The anti-inflammatory and antioxidant activities of the compounds were systematically evaluated utilising lipopolysaccharide-stimulated BV2 microglial cells. Anti-inflammatory activity was assessed by quantifying the mRNA expression levels of key pro-inflammatory cytokines [tumour necrosis factor-α, interleukin-1β, and interleukin-6 (IL-6)] via quantitative reverse transcription polymerase chain reaction. Among the synthesised derivatives, compounddemonstrated the most potent anti-inflammatory effect, exhibiting an ICvalue of 9.562 μM for the suppression of IL-6 expression and no significant cytotoxicity was observed. Notable, compoundexhibited better inhibitory potency against IL-6 expression compared to the anti-inflammatory drugs dexamethasone and Celecoxib. These findings strongly support the potential of compoundas a promising therapeutic candidate for mitigating secondary inflammation in SCI. 6g6g6g50

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Competing interests

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

PubMed

Abstract

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Funding

Competing interests

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