Spectrochimica acta. Part A, Molecular and biomolecular spectroscopySep 14, 2026

Imaging hydrogen sulfide-related oxidative imbalance in Parkinson’s disease models using near-infrared fluorescence and mass spectrometry

Longevity & Aging Weekly Brief ↗PubMed ↗DOI ↗

Updated Sep 15, 2026

Abstract

Enhanced NIR-II fluorescence signals were observed in the brains of MPTP-induced Parkinsonian mice, indicating H₂S-associated redox disruption.

The H₂S-responsive fluorophore CySO₃Cl exhibited fluorescence quenching in the presence of H₂S, providing a method to monitor H₂S-related changes.
In N2a cells, exposure to MPP⁺ resulted in reduced H₂S-associated redox status, as indicated by CySO₃Cl fluorescence responses.
Treatment with polyphenolic compounds, including resveratrol and tea polyphenols, led to decreased fluorescence signals and improved indicators of neuronal health in Parkinsonian models.
Ex vivo mass spectrometry imaging revealed decreased signals for dopamine and the H₂S-responsive product CySO₃SH in Parkinsonian mice, with partial recovery observed after treatment.
The imaging strategy employed may provide insights into redox dysregulation and treatment efficacy in Parkinson's disease.

Simplified

Hydrogen sulfide (H₂S) plays an important role in neuronal redox homeostasis and has been implicated in the regulation of dopaminergic neuronal injury, but its transient and reactive nature makes in vivo monitoring challenging. Herein, NIR-II fluorescence imaging coupled with mass spectrometry imaging (MSI) was employed to evaluate H₂S-associated redox dysregulation and treatment responses in Parkinsonian models. The H₂S-responsive fluorophore CySO₃Cl showed fluorescence quenching after reaction with H₂S, thereby providing an inverse optical readout of H₂S-related changes. In N2a cells, CySO₃Cl responded to both exogenous and endogenously modulated H₂S and revealed reduced H₂S-associated redox status in MPP⁺-induced neuronal injury. In MPTP-induced Parkinsonian mice, enhanced NIR-II fluorescence signals were observed in the brain region, indicating H₂S-associated redox disruption. Treatment with polyphenolic compounds, especially resveratrol and tea polyphenols, decreased the fluorescence signal and improved pathological, behavioral, and oxidative stress-related indices, suggesting partial recovery of H₂S-associated redox homeostasis. Furthermore, ex vivo MSI provided complementary spatial molecular information by mapping CySO₃Cl, its H₂S-responsive product CySO₃SH, and dopamine in brain tissues. The MSI results showed reduced dopamine and CySO₃SH signals but increased residual CySO₃Cl signals in Parkinsonian mice, which were partially reversed after polyphenolic treatment. Together, this study presents a coupled NIR-II fluorescence/MSI imaging strategy for evaluating H₂S-associated redox dysregulation and treatment responses in Parkinsonian models.

Full Text

Full text is available at the source.

View Full Text ↗

Imaging hydrogen sulfide-related oxidative imbalance in Parkinson’s disease models using near-infrared fluorescence and mass spectrometry

Abstract

Full Text

You found one interesting study. We’ll send the next 7.

What Lands in Your Inbox Each Week:

Recent issues from the longevity & aging brief