Parabacteroides goldsteinii enriched by Pericarpium Citri Reticulatae ‘Chachiensis’ polysaccharides improves colitis via the inhibition of lipopolysaccharide-involved PI3K-Akt signaling pathway

Jul 31, 2024International journal of biological macromolecules

Polysaccharides from Pericarpium Citri Reticulatae 'Chachiensis' increase Parabacteroides goldsteinii to reduce colitis by blocking inflammation signaling

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Abstract

Polysaccharides from Pericarpium Citri Reticulatae 'Chachiensis' (PCRCP) significantly reduced colitis in rats and mice induced by dextrose sodium sulfate (DSS).

Gut microbiota dysbiosis is linked to a higher incidence of colitis.
PCRCP treatment enhanced colonic barrier integrity and reduced inflammatory signaling pathways associated with colitis.
The protective effects of PCRCP against DSS-induced injuries were partially due to changes in gut microbiota.
Enrichment of the gut commensal bacterium Parabacteroides goldsteinii was observed following PCRCP treatment.
Administration of Parabacteroides goldsteinii alleviated colitis symptoms and inhibited inflammatory responses in DSS-exposed mice.
Activation of the PI3K-Akt signaling pathway negated the anti-colitic effects of Parabacteroides goldsteinii.

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Epidemiological and preclinical studies have indicated a factual association between gut microbiota dysbiosis and high incidence of colitis. Dietary polysaccharides can specifically shift the composition of gut microbiome response to colitis. Here we validated the preventive role of polysaccharides from Pericarpium Citri Reticulatae 'Chachiensis' (PCRCP), a well-known traditional Chinese medicine, in colitis induced by dextrose sodium sulfate (DSS) in both rats and mice. We found that treatment with PCRCP not only significantly reduced DSS-induced colitis via down-regulating colonic inflammatory signaling pathways including PI3K-Akt, NLRs and NF-κB, but also enhanced colonic barrier integrity in rats. These protective activities of PCRCP against DSS-induced injuries in rats were in part due to the modulation of the gut microbiota revealed by both broad-spectrum antibiotic (ABX)-deleted bacterial and non-oral treatments. Furthermore, the improvement of PCRCP on colitis was impaired by intestinal neomycin-sensitive bacteria in DSS-exposed mice. Specifically, in vivo and in vitro treatment with PCRCP led to a highly sensible enrichment in the gut commensal Parabacteroides goldsteinii. Administration of Parabacteroides goldsteinii significantly alleviated typical symptoms of colitis and suppressed the activation of PI3K-Akt-involved inflammatory response in DSS-exposed mice. The anti-colitic effects of Parabacteroides goldsteinii were abolished after the activation of PI3K-Akt signaling pathway by lipopolysaccharide treatment in mice exposed to DSS. This study provides new insights into an anti-colitic mechanism driven by PCRCP and highlights the potential prebiotic of Parabacteroides goldsteinii for the prevention of ulcerative colitis.

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Parabacteroides goldsteinii enriched by Pericarpium Citri Reticulatae ‘Chachiensis’ polysaccharides improves colitis via the inhibition of lipopolysaccharide-involved PI3K-Akt signaling pathway

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