New reporter system for Per1 and Bmal1 expressions revealed self‐sustained circadian rhythms in peripheral tissues

Sep 27, 2006Genes to cells : devoted to molecular & cellular mechanisms

New system tracking Per1 and Bmal1 shows ongoing daily rhythms in body tissues

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Abstract

Circadian rhythms in Bmal1-FL in peripheral tissues persisted for more than 3 weeks.

  • A new reporter system was established to monitor the expression of Per1 and Bmal1 genes in cultured mouse tissue.
  • Circadian rhythms for Per1 and Bmal1 in the suprachiasmatic nucleus were robust and anti-phasic.
  • These rhythms were phase delayed by 4-8 hours compared to the corresponding transcript levels observed in vivo.
  • Circadian rhythms in peripheral tissues maintained a long-term oscillation despite external changes.
  • Refreshment of culture medium restored dampened circadian rhythms, but this restoration did not indicate a resetting of oscillation.

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