Photodiagnosis and photodynamic therapyJul 6, 2025

Light from 808 nm and 915 nm lasers may affect growth and bone-building of cheek fat stem cells

Regenerative Health Weekly Brief ↗PubMed ↗DOI ↗

Updated Apr 4, 2026

Abstract

PBM significantly enhanced cell proliferation, particularly at 4 J/cm² with the 808 nm laser and at 4 and 6 J/cm² with the 915 nm laser.

Buccal fat pad-derived stem cells showed increased proliferation in response to photobiomodulation therapy.
The expression levels of osteocalcin and osteopontin were upregulated in most groups treated with laser irradiation.
RUNX2 expression was elevated across all PBM-treated groups.
Alizarin red staining and alkaline phosphatase activity confirmed the stimulatory effects of PBM on osteogenic differentiation.

Simplified

AIM: Photobiomodulation therapy (PBM) is a promising adjunct in tissue engineering due to its ability to stimulate cell proliferation and differentiation. This study evaluated the effects of PBM on buccal fat pad-derived stem cells (BFPSCs) using two diode laser wavelengths.

METHODS: BFPSCs were divided into seven groups: six received laser irradiation and one served as control. Three groups were treated with a 915 nm laser and three with an 808 nm diode laser, each at energy densities of 2, 4, and 6 J/cm². Cell proliferation was assessed using the MTT assay, while osteogenic differentiation was evaluated by Alizarin red staining after 14 days. Alkaline phosphatase activity was measured after 7 days. Expression levels of osteocalcin (OCN), Osteopontin (OPN), and Runt-related transcription factor 2 (RUNX2) were measured by RT-PCR on days 7 and 14.

RESULTS: PBM significantly enhanced cell proliferation, particularly at 4 J/cm² (808 nm) and at 4 and 6 J/cm² (915 nm). Upregulation of OCN and OPN were detected in most irradiated groups. RUNX2 expression was higher in all PBM groups. Alizarin Red staining and alkaline phosphatase activity results confirmed PBM's stimulatory effects.

CONCLUSION: PBM, especially at 915 nm, enhances the proliferation and osteogenic differentiation of BFPSCs, suggesting potential benefits for bone reconstruction.

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Light from 808 nm and 915 nm lasers may affect growth and bone-building of cheek fat stem cells

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