Photodynamic inactivation of bacterial biofilms on absorbable collagen membranes using indocyanine green in aqueous and hydrogen peroxide solutions

Sep 10, 2025Lasers in medical science

Using light-activated indocyanine green with water or hydrogen peroxide to kill bacteria in biofilms on absorbable collagen membranes

AI simplified

Regenerative Health on OpenScience ↗PubMed ↗DOI ↗

Abstract

The combination of indocyanine green and hydrogen peroxide at minimum inhibitory concentration could significantly reduce bacterial viability in biofilms on collagen membranes.

Photodynamic therapy using indocyanine green and light treatment showed a notable reduction in bacterial presence.
The addition of hydrogen peroxide at minimum inhibitory concentration enhanced the antibacterial effect of the treatment.
Efficacy of the treatment was dependent on light activation; without irradiation, no significant bacterial reduction was achieved.
The combination approach may provide an effective method for managing biofilms on collagen membranes, reducing the need for high concentrations of hydrogen peroxide.

AI simplified

Microbial contamination of absorbable collagen membranes used in guided bone regeneration (GBR) may compromise healing outcomes. This study aimed to investigate whether the minimum inhibitory concentration (MIC) of hydrogen peroxide (HO) can improve the antibacterial effect of indocyanine green (ICG)-mediated antimicrobial photodynamic therapy (PDT) on absorbable collagen membranes while reducing the need for high HOconcentrations. A laboratory-based model was developed using Streptococcus sanguinis and Staphylococcus aureus. The biofilms were treated with ICG (1.29 mM), HO(at MIC), or their combination, followed by irradiation at 808 nm (12 J cm²) or no irradiation. The biofilm viability was assessed after treatment. PDT (ICG + 808 nm diode laser) showed a significant reduction of bacteria, which was further enhanced by the addition of HOat MIC concentrations. Although ICG-HO-PDT showed similar efficacy to 1 M HO, this effect was dependent on light activation. Without irradiation, no comparable result was observed, emphasizing that photoactivation is essential for achieving synergistic effects with HOat low concentration. This combination could effectively combat biofilms on collagen membranes and provide an alternative for infection control by reducing the dependence on high HOconcentrations. 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 -

Full Text

Full text is available at the source.

View full text (PDF) ↗View full text (HTML) ↗

Photodynamic inactivation of bacterial biofilms on absorbable collagen membranes using indocyanine green in aqueous and hydrogen peroxide solutions

Abstract

Full Text

You found one interesting study. We’ll send the next 7.

what lands in your inbox each week: