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Abstract
A novel biosensor, PINK1-SPARK, enables real-time monitoring of PINK1 activity in single live cells.
- PINK1 is involved in processes like mitophagy, cell death, and energy regulation within cells.
- Current methods provide limited insights into the dynamic and heterogeneous nature of PINK1 activity.
- PINK1-SPARK utilizes phase separation to report on PINK1 activity in real time.
- The biosensor allows for observation of PINK1 activity in response to mitochondrial stress and pharmacological agents.
- Multiplexed imaging with PINK1-SPARK can reveal relationships between PINK1 activity and mitochondrial damage.
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