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Expression of plasma membrane GABA transporters but not of the vesicular GABA transporter in dentate granule cells after kainic acid seizures
Changes in GABA transport proteins on cell surfaces but not in storage vesicles of dentate granule cells after kainic acid seizures
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Abstract
GAD67 mRNA levels increased by up to 300% in granule cells of the dentate gyrus after kainic acid-induced seizures.
- Kainic acid-induced seizures led to increased expression of GAD67 and GAD65 mRNA in granule cells within 6-24 hours.
- Increased GAD and GABA immunoreactivity was observed in the terminal field of mossy fibers and in presumed dendrites of granule cells.
- GAT-1 mRNA levels increased by 30% shortly after kainate injection but decreased by approximately 25% at later time points.
- GAT-3 mRNA was significantly reduced by 35-75% in granule cells from 4 hours to 30 days post-injection.
- No expression of the vesicular GABA transporter was detected in granule cells or mossy fibers.
- Increased expression of GAD and reduced expression of GABA transaminase may elevate cytoplasmic GABA concentrations in granule cells.
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