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Prediction and control of the particle size of polyethylene glycol–free lipid nanoparticles using a design of experiment
Predicting and controlling the size of lipid nanoparticles without polyethylene glycol using experimental design
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Abstract
Uniform lipid nanoparticles (LNPs) were formed with controlled particle size and polydispersity index (PdI) using a predictive model.
- PEG-free LNPs containing a pH-responsive lipid derivative were successfully prepared.
- The lipid solution ratio was identified as the most critical factor influencing particle size and PdI.
- ζ-potential and siRNA encapsulation efficiency were not influenced by the preparation conditions.
- A predictive model based on design of experiment (DoE) accurately forecasted the particle size of the LNPs.
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