Probiotics Exert Protective Effect against Sepsis-Induced Cognitive Impairment by Reversing Gut Microbiota Abnormalities

Dec 7, 2020Journal of agricultural and food chemistry

Probiotics may protect against sepsis-related thinking problems by restoring healthy gut bacteria

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Abstract

Probiotic Clostridium butyricum (Cb) significantly attenuated cognitive impairment and neuronal damage in a sepsis-associated encephalopathy mouse model.

Cognitive impairment was assessed using behavioral tests, showing notable improvement with Cb treatment.
Nissl and Fluoro-Jade C staining indicated reduced neuronal injury in mice treated with Cb.
Cb treatment significantly inhibited excessive activation of microglia and decreased levels of ionized calcium-binding adapter molecule 1 (Iba-1).
Increased levels of brain-derived neurotrophic factor (BDNF) were observed in the brains of SAE mice receiving Cb.
Analysis of gut microbiota through 16S rRNA sequencing revealed improvements in dysbiosis associated with SAE following Cb treatment.

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Recent evidence has revealed that probiotics could affect neurodevelopment and cognitive function via regulating gut microbiota. However, the role of probiotics in sepsis-associated encephalopathy (SAE) remained unclear. This study was conducted to assess the effects and therapeutic mechanisms of probiotic(Cb) against SAE in mice. The SAE model mouse was induced by cecal ligation and puncture (CLP) and was given by intragastric administration with Cb for 1 month. A series of behavioral tests, including neurological severity score, tail suspension test, and elevated maze test, were used to assess cognitive impairment. Nissl staining and Fluoro-Jade C (FJC) staining were used to assess neuronal injury. Microglia activation, the release of neuroinflammatory cytokines, and the levels of ionized calcium-binding adapter molecule 1 (Iba-1) and brain-derived neurotrophic factor (BDNF) in the brain were determined. The compositions of the gut microbiota were detected by 16S rRNA sequencing. Our results revealed that Cb significantly attenuated cognitive impairment and neuronal damage. Moreover, Cb significantly inhibited excessive activation of microglia, decreased Iba-1 level, and increased BDNF level in the SAE mice. In addition, Cb improved gut microbiota dysbiosis of SAE mice. These findings revealed that Cb exerted anti-inflammatory effects and improved cognitive impairment in SAE mice, and their neuroprotective mechanisms might be mediated by regulating gut microbiota. Clostridium butyricum

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Probiotics Exert Protective Effect against Sepsis-Induced Cognitive Impairment by Reversing Gut Microbiota Abnormalities

Abstract

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