New pyrazole-linked thiophene compounds made by Gewald synthesis as antibacterial and anti-inflammatory agents

Compounds 8b, 8c, 8f, 8g, 8k, 8n, and 8o exhibited promising antibacterial activity.

• Compounds were synthesized from 2-amino-5-(2,4-dichlorophenyl)thiophene-3-carbonitrile and 1,3-disubstituted-1H-pyrazole-4-carbaldehydes.
• In vitro screening demonstrated antibacterial and anti-inflammatory activities of the synthesized compounds.
• Compound 8a showed potential as an antitubercular agent against Mycobacterium tuberculosis H37Rv.
• Hemolytic assays indicated that the compounds are nontoxic, with hemolysis percentages between 3.6 and 20.1 at a concentration of 1 mg/ml.
• The substitution pattern on the pyrazole ring may influence the biological activity of the compounds.

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