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Abstract
Coculturing tumor cells and macrophages induced increased presentation of peptides derived from proteins associated with cytokine signaling pathways on macrophages.
- Immune checkpoint inhibitors are generally ineffective in glioblastoma due to the immunosuppressive environment created by GBM-associated macrophages.
- Cell type-specific immunopeptidome analysis revealed distinct MHC-I-associated antigens at the tumor-macrophage interface.
- Coculture systems showed elevated presentation of specific peptides linked to cytokine signaling on macrophages and Rho GTPase pathways on GBM tumor cells.
- An mRNA vaccine encoding six peptides from GAMs and GBM tumor cells was developed.
- Vaccination with two doses produced an antigen-specific immune response and significantly delayed GBM tumor growth, with some cases resulting in tumor eradication.
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