RBM14 is indispensable for pluripotency maintenance and mesoderm development of mouse embryonic stem cells

May 6, 2018Biochemical and biophysical research communications

RBM14 is essential for keeping mouse embryonic stem cells able to become any cell type and for forming early muscle and bone tissue

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Abstract

RBM14 is essential for maintaining pluripotency and mesoderm development in mouse embryonic stem cells (mESCs).

RBM14 is expressed in the nucleus of mESCs and is a component of the para-speckle complex.
Depletion of RBM14 using CRISPR technology led to altered gene expression profiles in mESCs.
Pluripotency-associated genes and genes related to Wnt and TGF-β signaling pathways were downregulated following RBM14 knockout.
RBM14 is crucial for mesoderm development both in vitro and in vivo.
The specific effects of RBM14 depletion were confirmed through a rescue experiment.

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The pluripotency of embryonic stem cells (ESCs) is maintained by core pluripotency transcription factors, cofactors and several signaling pathways. RBM14 is a component of the para-speckle complex, which has been implicated in multiple important biological processes. The role of RBM14 in ESCs and lineage differentiation remains to be elucidated. In the present study, we provided evidence that RBM14 plays important roles in maintaining pluripotency and in the early differentiation of ESCs. RBM14 was demonstrated to be expressed in mouse embryonic stem cells (mESCs) and localized in the nucleus. RBM14 expression was depleted in mESCs using clustered regularly interspaced short palindromic repeats (CRISPR) technology. Our results also showed that RBM14 depletion altered the gene expression profiles of mESCs. In particular, pluripotency-associated genes and genes involved in the Wnt and TGF-β signaling pathways were downregulated in RBM14 knockout mESCs. Furthermore, RBM14 was found to be essential for mesoderm development in vitro and in vivo. The specific effects of RBM14 depletion were verified by conducting a rescue experiment. Our findings demonstrated that RBM14 not only plays an important role in maintaining the pluripotency of mESCs but is also indispensable for mesoderm development.

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RBM14 is indispensable for pluripotency maintenance and mesoderm development of mouse embryonic stem cells

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