Artificial cells, nanomedicine, and biotechnologyJul 29, 2026

Recent progress in CRISPR gene editing tools and delivery methods

mRNA Technology Weekly Brief ↗PubMed ↗DOI ↗OA ↗

Updated Aug 3, 2026

Abstract

Developing optimized delivery systems is crucial for realizing the full potential of CRISPR technology.

Current delivery strategies include biological methods like AAV, VLP, SEND, and chemical methods such as LNP.
Each delivery system has limitations related to editing efficiency, immunogenicity, and half-life.
A systematic review was conducted to analyze the types, principles, and characteristics of gene editing systems.
The review focuses on factors such as type, principle, load, immunogenicity, specificity, and toxicity of delivery systems.
Insights from this review could help facilitate the selection of appropriate delivery systems for CRISPR applications.

Simplified

CRISPR technology is a powerful tool for gene editing, in which the efficient delivery of living target cells allows it to show great clinical potential. At present, the commonly used in vivo delivery strategies mainly include biological methods (AAV, VLP, SEND) and chemical methods (LNP), which subtly deliver gene editors to living target cells safely and efficiently from different ways. However, existing delivery systems have different extents of limitations in terms of editing efficiency, immunogenicity, half-life, etc., so developing optimized delivery systems is the key to fully realizing the potential of CRISPR-Cas system for intracellular gene editing. In order to fully understand the advantages of different delivery strategies to maximize the ability to help CRISPR systems choose delivery methods, we conducted a systematic review. In this paper, we introduce the types, principles and characteristics of gene editing systems in order to understand their requirements for delivery tools. We focus on describing the type, principle, load, immunogenicity, specificity, toxicity, etc. of the delivery system, so as to fully analyse its advantages and disadvantages for the selection of different editing environments. This review aims to provide new insights to facilitate appropriate delivery systems or improve the efficacy of existing systems.

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Recent progress in CRISPR gene editing tools and delivery methods

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