Effects of resveratrol, a grape polyphenol, on catecholamine secretion and synthesis in cultured bovine adrenal medullary cells

Sep 25, 2007Biochemical pharmacology

Resveratrol from grapes and its effects on stress hormone release and production in cow adrenal cells

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Abstract

Resveratrol inhibited catecholamine secretion with an IC(50) of 20.4 microM in cultured bovine adrenal medullary cells.

Catecholamine secretion and synthesis were suppressed by resveratrol in a concentration-dependent manner.
The compound reduced sodium and calcium ion influx triggered by acetylcholine and other activators.
Resveratrol directly inhibited the current in neuronal nicotinic acetylcholine receptors expressed in Xenopus oocytes.
It also diminished catecholamine synthesis induced by acetylcholine at an IC(50) of 5.32 microM.
These findings suggest a mechanism where resveratrol may affect neurotransmitter release through ion channel modulation.

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We report the effects of resveratrol, a polyphenol found in the skins of red grapes, on catecholamine secretion and synthesis in cultured bovine adrenal medullary cells. Resveratrol suppressed catecholamine secretion and (22)Na(+) and (45)Ca(2+) influx induced by acetylcholine, an agonist of nicotinic acetylcholine receptors, in a concentration-dependent manner (IC(50)=20.4, 11.0, and 62.8 microM, respectively). Resveratrol also inhibited catecholamine secretion induced by veratridine, an activator of voltage-dependent Na(+) channels, and 56 mM K(+), an activator of voltage-dependent Ca(2+) channels, at concentrations similar to those for (45)Ca(2+) influx. Resveratrol directly inhibited the current evoked by acetylcholine in Xenopus oocytes expressing alpha3beta4 neuronal nicotinic acetylcholine receptors (IC(50)=25.9 microM). Furthermore, resveratrol (IC(50)=5.32 microM) attenuated (14)C-catecholamine synthesis induced by acetylcholine. The present findings suggest that resveratrol inhibits acetylcholine-induced catecholamine secretion and synthesis through suppressing ion influx in cultured bovine adrenal medullary cells.

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Effects of resveratrol, a grape polyphenol, on catecholamine secretion and synthesis in cultured bovine adrenal medullary cells

Abstract

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