Gene activity in the retina of two mouse strains to improve existing retinal data

RNA sequencing analysis generated over 30,000,000 reads per sample from the retinas of B6 and D2 mice.

• A total of 18,100 gene counts were identified across six retina samples.
• 1,665 genes were found to be differentially expressed between the B6 and D2 strains.
• Of these, 858 genes were more highly expressed in B6 retinas, while 807 were more highly expressed in D2 retinas.
• Differential activity was observed in several molecular pathways, including retinoic acid metabolism and the PI3K-Akt signaling pathway.
• The RNA-seq data showed a high correlation with microarray data, with Pearson's r values of 0.780 for B6 and 0.784 for D2 mice.
• The microarray data set demonstrated an overall accuracy of 91.1% in detecting differentially expressed genes.

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