In-depth understanding of human T-cell-mediated immunity in coronavirus disease 2019 (COVID-19) is needed if we are to optimize vaccine strategies and immunotherapies. Identification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) T-cell epitopes and generation of peptide-human leukocyte antigen (peptide-HLA) tetramers facilitate direct ex vivo analyses of SARS-CoV-2-specific T cells and their T-cell receptor (TCR) repertoires. We utilized a combination of peptide prediction and in vitro peptide stimulation to validate novel SARS-CoV-2 epitopes restricted by HLA-A24:02, one of the most prominent HLA class I alleles, especially in Indigenous and Asian populations. Of the peptides screened, three spike-derived peptides generated CD8IFNγresponses above background, S(QYIKWPWYI), S(NYNYLYRLF) and S(VFKNIDGYF), with Sgenerating immunodominant CD8IFNγresponses. Using peptide-HLA-I tetramers, we performed direct ex vivo tetramer enrichment for HLA-A24:02-restricted CD8T cells in COVID-19 patients and prepandemic controls. The precursor frequencies for HLA-A24:02-restricted epitopes were within the range previously observed for other SARS-CoV-2 epitopes for both COVID-19 patients and prepandemic individuals. Naïve A24/SARS-CoV-2-specific CD8T cells increased nearly 7.5-fold above the average precursor frequency during COVID-19, gaining effector and memory phenotypes. Ex vivo single-cell analyses of TCRαβ repertoires found that the A24/SCD8T-cell TCRαβ repertoire was driven by a common TCRβ chain motif, whereas the A24/SCD8TCRαβ repertoire was diverse across COVID-19 patients. Our study provides an in depth characterization and important insights into SARS-CoV-2-specific CD8T-cell responses associated with a prominent HLA-A24:02 allomorph. This contributes to our knowledge on adaptive immune responses during primary COVID-19 and could be exploited in vaccine or immunotherapeutic approaches. + + + + + + + + + + + 1208-1216 448-456 193-201 1208 448 1208
