Methods in molecular biology (Clifton, N.J.)

Features of Aging-Related Secretions in Natural and Lab-Grown Skin Cells Using Multiple Tests

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Abstract

The senescence-associated secretory phenotype (SASP) consists of a collection of inflammatory cytokines, chemokines, and growth factors released by senescent cells.

  • Senescent cells are characterized by growth arrest while remaining metabolically active.
  • Understanding SASP is important for insights into aging and age-related diseases such as cancer and Alzheimer's disease.
  • Investigating SASP may help ensure the safety and effectiveness of induced pluripotent stem cell (iPSC)-derived therapies in regenerative medicine.
  • A reproducible protocol for characterizing SASP in primary and iPSC-derived skin fibroblasts is provided through multiplex immunoassays.

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