Inhibition of reduced the formation and rupture of thoracic aortic dissections () in mice.
SGK1 is associated with the transformation of vascular smooth muscle cells from a contractile to a synthetic phenotype.
Pharmacological blockade of SGK1 led to decreased extracellular matrix degradation in blood vessels.
SGK1 promotes the degradation of SIRT6, which is involved in the regulation of expression.
SIRT6 transcriptionally inhibits MMP9 through epigenetic modification, indicating a regulatory axis involving SGK1, SIRT6, and MMP9.
The findings suggest that the SIRT6-MMP9 pathway may play a role in the development of TAD.
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BACKGROUND: The occurrence of thoracic aortic dissection () is closely related to the transformation of vascular smooth muscle cells (VSMCs) from a contractile to a synthetic phenotype. The role of (serum- and glucocorticoid-regulated kinase 1) in VSMC phenotypic transformation and TAD occurrence is unclear.
METHODS: Four-week-old male Sgk1(floxed) and Sgk1;Tagln(smooth muscle cell-specificknockout) mice were administered β-aminopropionitrile monofumarate for 4 weeks to model TAD. The SGK1 inhibitor GSK650394 was administered daily via intraperitoneal injection to treat the mouse model of TAD. Immunopurification and mass spectrometry were used to identify proteins that interact with SGK1. Immunoprecipitation, immunofluorescence colocalization, and GST (glutathione S-transferase) pull-down were used to detect molecular interactions between SGK1 and SIRT6 (sirtuin 6). RNA-sequencing analysis was performed to evaluate changes in the SIRT6 transcriptome. Quantitative chromatin immunoprecipitation was used to determine the target genes regulated by SIRT6. Functional experiments were also conducted to investigate the role of SGK1-SIRT6- (matrix metalloproteinase 9) in VSMC phenotypic transformation. The effect of SGK1 regulation on target genes was evaluated in human and mouse TAD samples. F/F F/F Cre Sgk1Sgk1
RESULTS: Sgk1;Taglnor pharmacological blockade of Sgk1 inhibited the formation and rupture of β-aminopropionitrile monofumarate-induced TADs in mice and reduced the degradation of the ECM (extracellular matrix) in vessels. Mechanistically, SGK1 promoted the ubiquitination and degradation of SIRT6 by phosphorylating SIRT6 at Ser338, thereby reducing the expression of the SIRT6 protein. Furthermore, SIRT6 transcriptionally inhibits the expression of MMP9 through epigenetic modification, forming the SGK1-SIRT6-MMP9 regulatory axis, which participates in the ECM signaling pathway. Additionally, our data showed that the lack of SGK1-mediated inhibition of ECM degradation and VSMC phenotypic transformation is partially dependent on the regulatory effect of SIRT6-MMP9. F/F Cre
CONCLUSIONS: These findings highlight the key role of SGK1 in the pathogenesis of TAD. A lack of SGK1 inhibits VSMC phenotypic transformation by regulating the SIRT6-MMP9 axis, providing insights into potential epigenetic strategies for TAD treatment.
Key numbers
37.5%
Decrease in Aortic Dissection Incidence
Observed in ;Tagln knockout mice after BAPN treatment.
60%
Increase in Expression
Detected in -deficient VSMCs compared to controls.
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