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SMG5–PNRC2 is functionally dominant compared with SMG5–SMG7 in mammalian nonsense-mediated mRNA decay
SMG5-PNRC2 plays a more important role than SMG5-SMG7 in mammalian mRNA quality control
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Abstract
PNRC2 preferentially interacts with SMG5, impacting mRNA degradation processes.
- Hyperphosphorylated Upf1 plays a crucial role in the recognition of substrates.
- Downregulation of PNRC2 disrupts the interaction between SMG5 and Dcp1a.
- Upf1, SMG5, and PNRC2 function at the same step in the NMD process.
- SMG6 is required for efficient mRNA degradation involving Upf1.
- There is a significant overlap between SMG5-dependent and PNRC2-dependent NMD substrates, suggesting a functional dominance of the SMG5-PNRC2 complex.
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Key numbers
2.3×
Decrease in mRNA degradation by Upf1 downregulation
Observed in tethering experiments with SMG5, SMG6, and PNRC2.
2.5×
Decrease in mRNA degradation by PNRC2 downregulation
Measured during tethering assays with SMG5 and Upf1.
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Overlap of transcripts upregulated by SMG5 and PNRC2
Identified in microarray analyses of transcript regulation.