Effects of sodium chloride on circadian period and temperature compensation of KaiC phosphorylation

Increasing sodium chloride (NaCl) concentrations progressively shortened the circadian period.

• The circadian clock generates ~ 24-h cycles in physiology and behavior, maintaining consistent timing across conditions.
• NaCl influences circadian rhythms, yet its exact effects on timing have been poorly understood.
• Higher concentrations of NaCl led to a decrease in circadian period without significantly changing the oscillation amplitude.
• While temperature compensation remained stable with varying levels of KaiB, increased NaCl concentrations partially disrupted this compensation.
• Changes in NaCl concentration correlated positively with Q₁₀ values, indicating an impact on temperature responses.
• NaCl may alter the balance of KaiB conformations and oligomerization, affecting circadian period and temperature compensation.

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