Effects of a Low-Energy Diet on Body Measurements and Heart Health Risks in Overweight and Obese Adults

Obesity is a complex, multifactorial condition defined as excessive fat accumulation that poses significant health risks, including increased susceptibility to cardiometabolic diseases such as type 2 diabetes, hypertension, and dyslipidemia [1]. The rate of obesity continues to increase globally, and it has more than doubled in adults since 1990, reaching a rate of 43% of adults who are overweight or obese in 2022 [2]. Unhealthy lifestyles, especially improper dietary habits besides physical inactivity, are major contributors to the development of obesity. Therefore, lifestyle advice, including an individual-adapted diet, remains a cornerstone for obesity treatment [1,2]. Consequently, alongside the increase in obesity incidence, the demand for effective dietary strategies that prevent weight gain, induce weight loss, and reduce the risk of chronic disease also increased [3]. An energy-restricted diet, usually targeting 20–30% of the estimated energy requirements of overweight or obese individuals, is one of the most commonly employed strategies for reducing body weight (BW) and body fat (BF) content [4,5]. The literature data confirmed the positive influence of energy-restricted diets on cardiometabolic risk factors, such as blood pressure, dyslipidemia, and glucose intolerance, which often improve after only a 5% loss of initial BW [6]. However, besides calorie restriction, manipulation in the nutrient composition of diet also has an influence on the process of weight loss and cardiometabolic risk factors, mostly relying on the well-established facts that alterations in macronutrient composition can influence endocrine balance and the gut microbiome, potentially affecting fat storage [1,7]. Several types of energy-restricted diets with altered macronutrient compositions have been examined for their effectiveness in promoting weight loss and improving cardiometabolic health [1,8]. Numerous studies have confirmed that carbohydrate-restricted diets positively impact weight loss and body composition, as well as improvements in blood glucose levels and lipid status, which are well-recognized cardiometabolic risk factors [9]. Low or moderate carbohydrate intake (less than 26% or 45% of overall energy intake, respectively) is often replaced with higher fat and protein consumption, which suppresses appetite by slowing gastric emptying and promoting prolonged satiety [10]. Namely, a high protein diet in which >20% of energy is derived is associated with greater satiety, reduced appetite, and preservation of lean body mass during reduced-calorie, low-fat diets [11,12]. Moreover, isocaloric diets that are lower in carbohydrates but higher in fat reduce insulin secretion since dietary fat does not stimulate insulin secretion. This reduction promotes fat loss from adipose tissue and makes free fatty acids available for use by metabolically active tissues [11]. Studies have shown that switching the diet from high carbohydrate to high fat (or vice versa) can impair the body’s ability to switch efficiently between glucose and fatty acid oxidation. This adaptability is critical for maintaining insulin sensitivity and metabolic health [13,14]. In addition, different macronutrient compositions, such as low-carbohydrate or high-fat diets, can significantly affect metabolic pathways and the body’s ability to utilize different fuel sources. This is particularly important in conditions such as obesity and type 2 diabetes, where metabolic inflexibility is common. Data from the literature also suggest that metabolic flexibility is associated with changes in the metabolism of muscle and adipose tissue, which are influenced by diet and other factors such as exercise [14]. However, energy-restricted diets may lead to adaptive thermogenesis, in which energy expenditure decreases more than would be expected from weight loss alone, hindering the sustainability of weight loss and promoting weight regain [15]. Data from the literature also suggest that strategic periods of higher calorie intake or intermittent fasting may prevent the metabolic adaptation typically observed with continuous energy restriction. This emphasizes the importance of alternating energy restriction and macronutrient metabolism to maintain fat loss and, thus, improve long-term weight loss outcomes [16]. Nonetheless, adherence to these energy-restricted and macronutrient-altered diets is a critical factor in their success [17]. Compliance tends to decrease over time due to various factors, such as biological, psychological, and socio-environmental factors related to eating, energy, and satiety [18]. Behavioral counseling to support lifestyle changes that counteract metabolic adaptations to weight loss shows promise for enhancing the success of weight loss interventions. For example, the inclusion of days off, a common practice in intermittent dieting, provided psychological flexibility and relief from constant restriction, which has been shown to increase adherence by reducing feelings of deprivation and promoting long-term adherence to the overall diet plan [4]. In addition, interventions that focus specifically on dietary factors such as protein and fiber can increase metabolic rate and offset the slight decrease in metabolism that accompanies weight loss [19]. One of the most effective strategies to increase adherence is regular counseling sessions, which significantly increase motivation to lose weight [20]. On the other hand, according to the study conducted by Trujillo-Garrido et al., one of the most common barriers to diet adherence was that patients were not prescribed a diet [20].

Anthropometric parameters are crucial in predicting cardiometabolic risk. While Body Mass Index (BMI) has traditionally been used as a primary measure, other parameters that are better indicators of general or central obesity have been shown to be superior in predicting cardiometabolic risk, such as waist circumference (WC), waist-to-hip ratio (WHR), and waist-to-height ratio (WtHR), and complement the use of BMI in identifying cardiovascular risk factors [21,22]. Moreover, besides anthropometric parameters, biochemical parameters should be considered for predicting cardiometabolic risk. Therefore, other predictive indexes for metabolic syndrome (MetS) have been developed, integrating both anthropometric parameters and biochemical factors as markers of adiposity [23,24].

The main aim of this study was to investigate the effects of an energy-restricted diet with alternating high, medium, and low carbohydrate intake on anthropometric parameters, biochemical markers, and cardiometabolic risk factors in overweight and obese adults. This intervention was designed to promote weight loss, improve body composition, and reduce cardiometabolic risks. Namely, the inclusion of anthropometric parameters (such as waist circumference, waist-to-hip ratio, and waist-to-height ratio) and biochemical markers (such as lipid levels and glucose) is essential for understanding the overall effect of our intervention on cardiometabolic health. These measurements were crucial to assessing how the alternative carbohydrate diet could improve both body composition and cardiometabolic risk. By examining both anthropometric and biochemical indices, this study aimed to provide a more comprehensive understanding of how the intervention could reduce cardiometabolic risk in overweight and obese individuals. This study also aimed to investigate the differential effects of the intervention on male and female participants and to assess whether gender-specific responses were observed in terms of fat loss, metabolic flexibility, and cardiometabolic improvements. To encourage participation in this study, regular counseling sessions were offered to personally guide and motivate participants throughout the duration of this intervention. We hypothesized that the diet intervention combined with structured counseling and days off would lead to improved diet adherence, significant reductions in body weight and fat mass, and improvements in anthropometric and biochemical markers of cardiometabolic health.

Obesity is a major global health problem that is closely associated with an increased risk of many diet-related, non-communicable diseases and mortality [30]. Obesity is characterized by excessive adipose tissue, resulting in dyslipidemia and disturbances in glucose metabolism, which are the major risk factors for the development of atherosclerosis and other cardiometabolic diseases [31]. Over the past two decades, numerous studies have been conducted on the effects of two dietary models, the calorie-restricted diet and the low-CH diet, on the reduction in body weight, especially fat mass, and the reduction in cardiometabolic risk factors.

With regard to the intake of CHs that characterized the days in our study, it is important to note that the intake of CHs is generally based on the established recommended intake of proteins and fats. Furthermore, a reduction in CH intake is always accompanied by a greater or lesser increase in protein intake in the diet, depending on how much additional fat needs to be included in the diet. The results of a meta-analysis from 2018 showed that a CH intake of around 40–70% was associated with a lower risk of death than a lower intake (<40%) or higher intake (>70%) [32]. However, in numerous clinical studies that have been conducted, a low-CH diet in which CH intake was reduced at the expense of increased protein intake (high-protein diet) or a high-fat diet (high-fat diet) in overweight adults had a beneficial effect on adipose tissue, triglyceride levels, serum glucose levels, and the inflammatory factor CRP [31,33,34]. One of the main problems of the very low-carbohydrate diet (<25% of energy intake) on which the keto diet is based is the difficulty of strictly adhering to this diet over a long period of time, as it restricts most foods characteristic of the Western diet, and its positive effect on serum total and LDL cholesterol is unclear [31]. There are certain safety concerns with low-CH diets, particularly diets with a total energy intake of <25%, including potential nutrient deficiencies that can occur when large and important food groups are restricted, the long-term effects of ketosis on bone and kidney health, and the possibility of hyperuricemia [9]. Precisely for these reasons, the dietary intervention in our study was mainly based on a medium CH intake, 40–49% of total energy intake, 5 days per week, while on one day per week, the CH intake was within the standard recommendations (up to 59%). A low-CH diet (<40% of daily energy intake) was recommended only once a week. Our decision to focus on a medium-carbohydrate intake for most of the intervention period was based on the existing literature that highlighted the safety and efficacy of this dietary approach. Namely, our results regarding the effects on lowering lipid parameters and fat mass are consistent with the results of Michalczyk et al. [31], who demonstrated that a low-calorie (20% relative to TDEE) and medium-CH diet (aproxx. 32% of energy intake) had a significant effect on lowering LDL, TG, and body fat. In addition, this diet led to an increase in HDL-C levels in overweight middle-aged men compared to a hypocaloric diet in which the ratio of macronutrients was not altered. Based on the observed beneficial effects in the treatment of obesity, the authors suggested this medium-CH diet as a dietary pattern for obese middle-aged men to effectively reduce adipose tissue while preserving muscle mass. However, in contrast to the study conducted by Michalczyk et al. [31], which lasted only 4 weeks, no statistically significant increase in HDL-C levels was observed in the subgroup of men in our study after 14 weeks of intervention, although the CH content was higher in our study, while the protein content was the same as in the above-mentioned study (about 30% of energy intake). The significant effect of diet on HDL-C levels was observed exclusively in the female subgroup. These results are consistent with the existing literature, which indicates that a low-carbohydrate diet can increase HDL-C levels by an average of 20% [35]. Remarkably, the increase in HDL-C levels was more pronounced in women than men, highlighting gender differences in lipoprotein response to diet [35,36]. The positive effect of diet on HDL-C levels is of great importance, as low HDL-C levels are recognized risk factors for cardiovascular disease and premature atherosclerosis, independent of triglyceride and LDL serum levels [35].

Calorie restriction is the most effective non-pharmacological measure that can lead to a reduction in body weight and prevent chronic metabolic diseases [37]. However, energy-restrictive diets can be problematic as they promote weight loss at the expense of loss of muscle mass and/or a reduction in resting energy expenditure, leading to increased hunger and compensatory motivation to overeat [37,38]. An additional risk factor that may be responsible for an inadequate response to a hypocaloric diet when it is used to reduce body weight is an increased hedonistic response to the sensory properties of foods, such as cravings for fatty, sweet, or salty foods that are normally excluded from the diet [39]. In addition, restrictive diets often trigger adaptive thermogenesis, in which the body reduces its resting metabolic rate and energy expenditure in response to prolonged calorie restriction. This leads to a slowdown in metabolism, which makes it increasingly difficult to maintain weight loss and can lead to weight regain over time [16]. Evidence from clinical studies suggests that increasing the protein content in a hypocaloric diet may promote satiety, probably due to the effect on appetite hormones, but may also lead to an increase in energy expenditure, as proteins have a greater thermic effect compared to fats and CHs [40]. In addition, the use of a hypocaloric diet with a higher protein content compared to the IOM’s stated acceptable macronutrient distribution range (AMDR) for protein (15–25% of total energy intake) [41] and had a more pronounced clinical effect on weight loss and fat mass compared to a diet with a standard amount of protein or a high-CH, low-fat diet [42]. Mateo-Gallego et al. [26] demonstrated that an energy-restricted diet with a high protein content (35% of total energy intake) led to greater fat loss and an improvement in cardiometabolic profile variables compared to a calorie-restricted diet with a lower protein intake (20% of total energy intake) in overweight and obese middle-aged women [26]. Positive effects of a high-protein diet (1.26 to 2.12 g/kg/d) on body composition have also been found in older men (>60 years), even without calorie restriction compared to controls [43]. Finally, adherence to a hypocaloric, high-protein, low-fat diet (32% protein, 12% fat) was more effective in promoting fat loss in both overweight men and women than adherence to a hypoenergetic diet with the same fat content but a protein content equivalent to the AMDR (22% protein, 12% fat) [44]. Consistent with the above research findings, this dietary program used in this study promoted effective loss of body weight, particularly fat mass, with a significant increase in the percentage of skeletal muscle mass in body weight compared to baseline and without the inclusion of planned physical activity. It should be noted that participants followed a diet 5 days a week that included the intake of 1.2–1.4 g/kg/day of protein, corresponding to a daily energy contribution of protein of 25–30% while consuming a standard amount of protein once a week (0.8–1.2 g/kg/day or 15–25% of energy intake). After the intervention, there was no increase in protein metabolic markers such as creatinine, urea, and blood uric nitrogen, which might indicate increased protein catabolism, while in the subgroup of women and people <40, blood uric nitrogen was significantly lower after the intervention.

Diets based on a lower than recommended carbohydrate intake are low in fiber, which largely determines the quality of the carbohydrates. Fiber intake is important for health, especially for gastrointestinal health and function, as well as for improved insulin sensitivity and weight regulation, while low-fiber diets are associated with various metabolic and gastrointestinal disorders [45,46]. Systematic reviews of randomized clinical trials and prospective observational studies in adults have shown that intake of at least 25 g of dietary fiber from fruits, vegetables, and cereals leads to beneficial improvements in obesity and non-communicable diseases with risk factors and is associated with lower risk of all-cause mortality [47]. The threshold of at least 25 g per day was chosen based on observational studies showing a dose–response relationship between dietary fiber intake and reduced risk of various non-communicable diseases and mortality. According to WHO recommendations, for 2000 kcal, the average daily intake of an adult about 25 g of dietary fiber should be consumed, which corresponds to an intake of 0.0125 g of dietary fiber per 1 kcal of energy intake [47]. Since 1 g of fiber has 2 kcal, it can be concluded that fiber should make up at least 2.5% of total energy intake. Since there is evidence that an increase in fiber intake to 50 g per day is likely to extend life expectancy and improve quality of life in the additional years [48], the fiber content in this dietary plan was adjusted to the energy intake, and the proportion of fiber in the total energy intake was kept constant at 5% (Table S1). This ensures that the daily intake of dietary fiber is 10 g and above, depending on the planned daily energy intake. The significant beneficial effects of the dietary regime implemented in this study on lipid parameters (lowering LDL cholesterol and TG and increasing HDL-C) and fasting blood glucose can be explained by planning an adequate intake of dietary fiber even on days when CH intake was <40%. Similarly, in a study conducted by Michalczyk et al., in which they observed the effects of a medium-CH diet in obese middle-aged men, fiber intake was 24.6 ± 2.6 g [31]. Fiber intake may also be responsible for the feeling of satiety reported by the subjects, which, together with a higher protein intake, allowed for better adherence and satisfaction with the prescribed dietary regimen. In addition, promoting the consumption of dietary fiber is crucial in the context of weight loss, as it increases the feeling of satiety, improves fat oxidation, and regulates metabolism. Fiber promotes the growth of beneficial gut bacteria that produce short-chain fatty acids (SCFAs), which help control appetite, increase insulin sensitivity, and reduce inflammation. This positive influence on the gut microbiota contributes to more effective weight control and reduces the metabolic problems associated with obesity [49,50].

With regard to fat intake, the dietary intervention in this study was planned in a way that fat intake followed the change in protein and carbohydrate intake. For example, on days when protein intake was highest and carbohydrate intake was lowest, fats accounted for up to 35% of total energy intake. On medium CH days, fat intake corresponded to AMDR and was in the range of 25–30%, while on maximum CH days, once a week, fat intake could be below AMDR. In studies comparing the effects of a low-carbohydrate diet with a low-fat diet in obese people, the low-carbohydrate diet was found to be more effective in lowering total and LDL-C cholesterol, lowering TG, and increasing HDL levels [51,52]. An important factor influencing the effectiveness of the low-CH diet was the quality of the fats, i.e., a more pronounced effect on the lipid profile was achieved when saturated fats were replaced by monounsaturated and polyunsaturated fats [33,53]. In the diet used in this study, the saturated fat content was <10% of energy intake, which is in line with WHO recommendations [54].

One of the peculiarities of our dietary regime to increase adherence to the diet is the introduction of a day off after three cycles of 3 weeks of hypocaloric diet, on which patients were allowed to consume food ad libitum. The conclusions of this study comparing the success of the 12-month application of the different diet programs (Atkins, Zone, and Ornis) in reducing body weight in obese people were that the effectiveness of the diet depended primarily on adherence to the prescribed diet program and that it was necessary to put more emphasis on strategies to increase adherence in order to increase the effect of weight loss [55]. Studies comparing the effectiveness of calorie-restricted diets and intermittent or alternate-day fasting have also shown that adherence to diets where food was allowed ad libitum on certain days or at certain times was higher [56]. The results of the questionnaire we administered to participants showed very high satisfaction with the diet and a low score for participants’ intention to stop the diet during this study, resulting in high adherence to the diet (Figure S1).

In order to verify the success of the dietary intervention in overweight and obese participants in terms of cardiometabolic risk reduction, the effects of the regimen were also evaluated using specific indices of body composition and/or adiposity that integrated anthropometric and biochemical parameters and, thus, represented useful and reliable indices for the early detection of cardiometabolic risks, particularly MetS. Although there was a significant decrease in all anthropometric parameters and indices, VAI and CMI are reported in the literature as the most informative indices that can predict cardiometabolic risks with high reliability, especially the occurrence of MetS in obese people, particularly in obese women [24]. The VAI is based on two anthropometric parameters (WC and BMI), two biochemical factors (TG and HDL), and the cardiometabolic index (CMI), which integrates the marker for obesity, the WtHR, with the TG/HDL-C ratio. Based on the CMI and VAI values obtained after the 14-week intervention, we can conclude that the number of participants with cardiometabolic risks was halved, with no significant difference between the different subgroups. Furthermore, based on the AIP value obtained after the end of the intervention, the number of participants with a risk for atherosclerosis decreased more than three times. Namely, given the critical role of triglycerides and cholesterol in the pathogenesis of atherosclerotic and cardiovascular diseases, the atherogenic lipoprotein profile of plasma has been identified as a significant risk factor for atherosclerosis [57]. The Atherogenic Index of Plasma, quantitatively defined as the logarithm of the ratio of triglycerides to HDL-C, has emerged as a robust predictor of future cardiovascular events, including coronary artery disease and other cardiovascular diseases [29,58]. Consequently, AIP monitoring can be used together with other parameters to assess the effectiveness of intervention programs to reduce cardiovascular risk [57]. According to the calculated AIP value and the proposed cut-off value for moderate (0.1–0.24) and high risk (>0.24) of cardiovascular disease, there were significant differences between male and female participants in our study, with men having a significantly higher AIP value and diet having a significantly stronger influence on AIP in men. Higher AIP values in the male population were also observed by Dobiásová et al. [59]. In addition, according to the study by Westphal-Nardo et al. [57], AIP proved to be a very valuable tool for predicting cardiometabolic risk in male participants.

Diet adherence is critical to the success of weight loss programs [20]. In this study, several strategies were used to improve diet adherence. These included telephone counseling, a prescribed diet, and ongoing self-monitoring. All of these strategies were found to be effective in promoting long-term diet adherence and successful weight management. Regular contact with healthcare professionals via counseling has been shown to reduce dropout rates and improve diet adherence by providing personalized feedback and addressing challenges that arise [17,20]. In addition, participants in our study received structured meal plans tailored to their needs. Structured diets reduce the cognitive load associated with daily food choices and make it easier for participants to stick to this program. Research shows that clear and structured dietary guidelines are essential for improving adherence, as they reduce confusion and promote adherence [17,20]. In addition, regular follow-up allowed participants to track their progress and receive ongoing feedback. Self-monitoring is widely recognized as one of the most effective strategies to improve adherence to dietary interventions, as it promotes accountability and facilitates timely adjustments [17]. Participants in our study reported their dietary adherence twice a week, which further enhanced their engagement with the intervention and allowed them to receive real-time feedback from medical staff. The literature also emphasizes the importance of tailoring nutritional interventions to individual preferences and dietary needs. This personalization ensures that diets are more sustainable and enjoyable for participants, which is crucial for long-term adherence [60]. In addition, strategies to combat binge eating, such as inducing ketosis through low-carbohydrate days or introducing an ad libitum day, have been shown to suppress cravings and improve long-term adherence to an energy-restricted diet [60].

However, the strategy of alternating carbohydrate intake, coupled with days off and a daily alternating the extent of energy deprivation, was designed to prevent metabolic adaptation. This approach allowed participants to maintain fat oxidation while reducing the compensatory responses that often accompany continuous calorie restriction. By alternating between low, medium, and high carbohydrate intake days, we provided metabolic flexibility, which is essential for maintaining long-term fat loss and avoiding weight plateaus [4,13,14]. While the results of this study provide valuable insights into the potential benefits of a controlled dietary intervention on body composition and cardiometabolic health, several important factors must be considered. First, it should also be noted that the diet used in this study included a structured alternation of carbohydrates, fats, and proteins. The proportion of these macronutrients varied from day to day, with days of minimum, medium, and maximum carbohydrate intake. Therefore, the results observed in this study may be due to the combined effects of changes in carbohydrate, fat, and protein intake rather than carbohydrate intake alone. The relatively small sample size and lack of a control group limit the ability to generalize the results. Without a comparison group, it is difficult to determine whether the observed improvements are specific to the alternative carbohydrate intake or solely due to calorie restriction. Future studies should include randomized control groups adhering to standard dietary interventions to better isolate the effects of this dietary strategy. In addition, the self-selected composition of the participant cohort could lead to selection bias, as individuals who were more motivated to adopt a healthier lifestyle were more likely to volunteer. This may have contributed to the high adherence rates and limited the external validity of the results. Future studies should aim to include a more randomized and representative population to ensure broader applicability of the results. The relatively short duration of the 14-week intervention limits our ability to assess the long-term sustainability of the dietary approach. Although significant improvements in weight loss and cardiometabolic markers were observed in the short term, weight regain and metabolic adjustment are common after dietary interventions. Longer-term follow-up studies are needed to determine whether the benefits observed during the intervention can be maintained over time. One of the greatest strengths of this study was the integration of regular counseling sessions with healthcare professionals and dietitians, which likely played a critical role in promoting adherence and achieving the observed results. The professional support not only helped participants adhere to the dietary protocol but also provided them with personal guidance that strengthened their commitment to this program. However, it is important to realize that constant access to healthcare professionals is not always possible for individuals in the real world, which could limit the generalizability of the results in settings without such support. Nonetheless, this highlights the crucial role that healthcare professionals and dietitians play in helping people achieve and maintain their nutrition and health goals.

This study showed that a specially designed energy-restricted diet with alternating carbohydrate intake led to a significant reduction in body weight, with more than half of the participants achieving a weight loss of at least 10%. The average weight loss in the entire group was 17%, with a particular increase in the proportion of muscle mass in relation to total body weight. These results underline the effectiveness of the diet in overweight and obese individuals. In addition, the intervention improved important cardiometabolic risk factors, including reductions in fasting blood glucose, LDL cholesterol, and predictive indices of cardiometabolic risk. Gender differences were observed, particularly in HDL-C levels, where the intervention had different effects in men and women, indicating possible gender-specific responses to the diet. The use of alternate days for carbohydrate intake combined with tailored counseling and continuous self-monitoring effectively improved adherence to the diet intervention. This multifaceted approach improved metabolic flexibility and reduced the likelihood of metabolic adaptation, which contributed to sustained fat loss and improved cardiometabolic health. While the dropout rate (65 out of 100 participants completed this study) was comparable to similar studies with intensive monitoring, we believe that the inclusion of structured support mechanisms—such as regular counseling sessions and continuous self-monitoring—helped to reduce the higher dropout rates. While these interventions did not completely prevent participants from dropping out of this study, they helped to ensure that participants stayed with this study for its duration. However, the relatively short duration of this study and the lack of a control group limited the ability to draw long-term conclusions. Future studies should aim to replicate these results with larger, more diverse populations, include control groups, and extend the intervention period to better assess the long-term sustainability and efficacy of this dietary approach.

This work has led to the development of a patent titled “Diet Composition for Body Weight Reduction”, with the following details: Inventor: Ana Petrovic; Patent Number: 63407; Application Number: P-2021/0019; Issuing Organization: The Intellectual Property Office of the Republic of Serbia; Year of Issue: 2022; State: Republic of Serbia.

We would like to express our sincere gratitude to the reviewers for their insightful comments and suggestions, which have greatly contributed to improving the quality and clarity of this manuscript.

The following supporting information can be downloaded at https://www.mdpi.com/article/10.3390/nu16203453/s1↗, Figure S1. Satisfaction questionnaire performed on participants after 14 weeks of dietary intervention; Table S1. Example of a meal plan for 1 cycle for one participant; Table S2. Macronutrient composition of the diet using the example of a participant; Table S3. Examples of changes in a participant’s anthropometric parameters during the dietary intervention.

Conceptualization, A.P. and N.D.I.; methodology, A.P.; software, S.J., N.M. and M.D.; validation, A.P., N.M. and N.D.I.; formal analysis, N.M. and N.D.I.; investigation, A.P.; resources, A.P.; data curation, N.M., M.D. and N.D.I.; writing—original draft preparation, A.P. and N.D.I.; writing—review and editing, B.D., N.M., S.J. and M.D.; visualization, N.D.I. and B.D.; supervision, N.D.I. and N.M.; project administration, A.P.; funding acquisition, A.P. All authors have read and agreed to the published version of the manuscript.

This study was conducted in accordance with the Declaration of Helsinki and approved by the Ethics Committee of the Faculty of Pharmacy, University of Belgrade (approved on 13 May 2023, Approval ID: 2891/2), Serbia. Participation was voluntary and informed consent was obtained from all participants who agreed to participate in this study. The data collection was anonymous, voluntary, and without requests for the respondents’ personal data.

Informed consent was obtained from all subjects involved in this study.

The data presented in this study are available upon request from the corresponding author due to ethical restrictions.

Author Ana Petrovic was employed by the company “Nutritional Studio Ana Petrovic”. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

This research received no external funding.