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Effects of subacute ruminal acidosis on colon epithelial morphological structure, permeability, and expression of key tight junction proteins in dairy goats
Subacute ruminal acidosis may change colon lining structure, leakiness, and key barrier proteins in dairy goats
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Abstract
Grain-induced subacute ruminal acidosis (SARA) is associated with profound disruption of the colonic epithelium in dairy goats.
- The electron density of tight junctions in the colonic epithelium decreased, while intercellular spaces widened and mitochondria swelled in goats experiencing SARA.
- In SARA goats, the colon's short-circuit current and tissue conductance increased, while the potential difference decreased compared to control goats.
- The mucosal-to-serosal flux of fluorescein isothiocyanate-dextran 4 kDa was elevated in SARA goats, indicating increased permeability.
- SARA led to increased mRNA and protein expression levels of tight junction proteins CLDN1 and OCLN in the colonic epithelium.
- Overall, SARA impaired the barrier function of the colonic epithelium at both structural and functional levels.
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