Super-resolution imaging of multiple organelle interactions between lysosome-lipid droplets-mitochondria

The novel fluorescent probe BDPQ enables precise quantification of cellular processes with minimal spectral bleed-through.

• BDPQ demonstrates low toxicity, high cell permeability, and superior photostability compared to commercial dyes.
• The developed 3D tri-color super-resolution technique allows for detailed observation of lipophagy, mitophagy, and their coordination in single cells.
• Starvation leads to low-level coordinated lipophagy and mitophagy, which may help sustain cell viability.
• Mitochondrial damage is associated with high-level autophagy and apoptosis.
• 2000 kDa hyaluronic acid could significantly enhance coordinated lipophagy and mitophagy, resulting in lipid droplet depletion and apoptosis.

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