Advances in tissue engineering of vasculature through three‐dimensional bioprinting

Jun 11, 2021Developmental dynamics : an official publication of the American Association of Anatomists

Progress in creating blood vessel tissues using 3D bioprinting

AI simplified

Regenerative Health on OpenScience ↗PubMed ↗DOI ↗

Abstract

3D bioprinting offers various techniques for the fabrication of vascularized tissue constructs and vessel-like structures.

Two main types of vasculature constructs are vascularized tissue constructs and vessel-like tubular structures.
Both direct and indirect printing methods can be utilized in the engineering of vascularized tissues.
Direct printing allows for simultaneous structural fabrication and cell seeding, while indirect printing is better for creating complex architectures.
Techniques such as extrusion bioprinting, inkjet bioprinting, and light-assisted bioprinting can be selectively applied to optimize tissue structure.
The selection of appropriate cells and biomaterials is crucial for achieving successful fabrication of specific vasculature constructs.
Despite advancements, 3D bioprinting remains in its early stages, with significant gaps between laboratory tests and real-world applications.

AI simplified

BACKGROUND: A significant challenge facing tissue engineering is the fabrication of vasculature constructs which contains vascularized tissue constructs to recapitulate viable, complex and functional organs or tissues, and free-standing vascular structures potentially providing clinical applications in the future. Three-dimensional (3D) bioprinting has emerged as a promising technology, possessing a number of merits that other conventional biofabrication methods do not have. Over the last decade, 3D bioprinting has contributed a variety of techniques and strategies to generate both vascularized tissue constructs and free-standing vascular structures.

RESULTS: This review focuses on different strategies to print two kinds of vasculature constructs, namely vascularized tissue constructs and vessel-like tubular structures, highlighting the feasibility and shortcoming of the current methods for vasculature constructs fabrication. Generally, both direct printing and indirect printing can be employed in vascularized tissue engineering. Direct printing allows for structural fabrication with synchronous cell seeding, while indirect printing is more effective in generating complex architecture. During the fabrication process, 3D bioprinting techniques including extrusion bioprinting, inkjet bioprinting and light-assisted bioprinting should be selectively implemented to exert advantages and obtain the desirable tissue structure. Also, appropriate cells and biomaterials matter a lot to match various bioprinting techniques and thus achieve successful fabrication of specific vasculature constructs.

CONCLUSION: The 3D bioprinting has been developed to help provide various fabrication techniques, devoting to producing structurally stable, physiologically relevant, and biologically appealing constructs. However, although the optimization of biomaterials and innovation of printing strategies may improve the fabricated vessel-like structures, 3D bioprinting is still in the infant period and has a great gap between in vitro trials and in vivo applications. The article reviews the present achievement of 3D bioprinting in generating vasculature constructs and also provides perspectives on future directions of advanced vasculature constructs fabrication.

Full Text

Full text is available at the source.

View full text (PDF) ↗View full text (XML) ↗View full text ↗

Advances in tissue engineering of vasculature through three‐dimensional bioprinting

Abstract

Full Text

You found one interesting study. We’ll send the next 7.

what lands in your inbox each week:

Abstract

Full Text

Related papers

You found one interesting study. We’ll send the next 7.

what lands in your inbox each week: