Profiling RNA changes from very small sample amounts

Uli-epic is a library construction strategy for profiling RNA modifications from ultra-low-input samples.

This platform experiment applied Uli-epic BID-seq to pseudouridine sites using 500 pg of rRNA-depleted mouse neural stem-cell and sperm RNA, and Uli-epic GLORI to quantify mA using 10 ng of rRNA-depleted RNA.

The abstract gives limited validation detail and demonstrates the method only in selected mouse sample types.