VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway

Nov 1, 2021Bioengineered

VAP-PLGA microspheres help fat-derived stem cells heal chronic skin wounds in mice through the PI3K/Akt/HIF-1α pathway

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Regenerative Health on OpenScience ↗PubMed ↗DOI ↗OA ↗

Abstract

Velvet antler polypeptide (VAP) significantly enhanced the proliferation and migration of (ADSCs).

VAP is associated with improved angiogenesis in human umbilical vein endothelial cells (HUVECs).
ADSCs treated with VAP showed increased levels of key signaling proteins related to cell growth and migration.
The combination of VAP with PLGA microspheres further enhanced the healing effects of ADSCs on .
VAP-PLGA treatment resulted in increased levels of vascular endothelial growth factor (VEGF) and stromal cell-derived factor-1 (SDF-1) in wound tissues.
Reduction of inflammatory markers such as interleukin-1 β (IL-1β) and IL-6 was observed in wound tissues after VAP-PLGA treatment.

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are a primary global health problem. Velvet antler polypeptide (VAP) regulates endothelial cell migration and angiogenic sprout. (ADSCs) are reported to make pivotal impacts upon wound healing. This study aimed to explore the role of VAP combined with ADSCs in wound healing of chronic skin ulcers. The effect of VAP on phenotypes of ADSCs, and VAP (PLGA microspheres) combining with ADSCs on wound healing of chronic skin ulcerswas evaluated. VAP generally promoted the proliferation, migration and invasion of ADSCs, and ADSC-induced angiogenesis in human umbilical vein endothelial cells (HUVECs) through . VAP-PLGA (PLGA microspheres) enhanced the promoting effect of ADSCs on wound healing, pathological changes, and angiogenesis in chronic skin ulcers. VAP-PLGA intensified the effect of ADSCs on up-regulating the levels of p-PI3K/PI3K, p-Akt/Akt, HIF-1α, vascular endothelial growth factor (VEGF), stromal cell-derived factor-1 (SDF-1), C-X-C motif chemokine receptor 4 (CXCR4), angiopoietin-4 (Ang-4), VEGF receptor (VEGFR), and transforming growth factor-β1 (TGF-β1), and down-regulating the levels of interleukin-1 β (IL-1β), IL-18 and IL-6 in wound tissues in chronic skin ulcers. Collectively, VAP promoted the growth, migration, invasion, and angiogenesis of ADSCs through activating PI3K/Akt/HIF-1α pathway, and VAP-PLGA enhanced the function of ADSCs in promoting wound healing, which was associated with angiogenesis, inflammation inhibition, and dermal collagen synthesis. in vivo in vivo in vivo in vivo

Key numbers

14 days

Wound healing rate increase

Wound healing rate observed on the 14th day post-treatment

IL-1β, IL-18, IL-6

Inflammatory cytokine reduction

Cytokines levels decreased in and VAP-PLGA groups

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VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway

Abstract

Key numbers

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