Analysis of Volatile Components in Curcuma Rhizome by Microemulsion Electrokinetic Chromatography

Jul 25, 2012Journal of chromatographic science

Analysis of volatile compounds in turmeric root using microemulsion electrokinetic chromatography

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Abstract

The developed MEEKC method successfully separated four volatile compounds from Curcuma rhizome within 30 minutes.

Germacrone, furanodiene, curcumenol, and curdione were identified as key volatile components in Curcuma rhizome.
The method utilized a running buffer with specific concentrations of sodium dodecyl sulfate, 1-octane, 1-butanol, and propanol.
Experimental parameters such as pH and electrolyte composition were optimized for effective separation.
The MEEKC method may serve as a valuable tool for analyzing heat-sensitive compounds in natural products.

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Volatile chemicals are a group of very important compounds in natural products. Curcuma rhizome, which contains many bioactive volatile compounds, is a traditional Chinese medicine that has long been used for the treatment of several diseases. In the present study, a microemulsion electrokinetic chromatography (MEEKC) method was developed for the analysis of four volatile components in Curcuma rhizome, including germacrone, furanodiene, curcumenol and curdione. Experimental parameters, including the pH, type and concentrations of background electrolyte, and microemulsion compositions (type and concentrations of surfactant, co-surfactant and oil phase) were intensively investigated. Finally, the primary compounds in the methanol extract of Curcuma rhizome were separated within 30 min using a running buffer composed of 2.31% w/v (80 mmol/L) sodium dodecyl sulfate (SDS), 0.91% w/v (80 mmol/L) 1-octane, 6.95% w/v (937.5 mmol/L) 1-butanol and 1.88% w/v (312.5 mmol/L) propanol in a 5-mM borate buffer (pH 8.1). The contents of the four investigated compounds were determined in the rhizome from C. phaeocaulis. The results showed that the developed MEEKC method provided an alternative tool for the analysis of volatile components, especially those of heat-sensitive compounds from natural products.

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Analysis of Volatile Components in Curcuma Rhizome by Microemulsion Electrokinetic Chromatography

Abstract

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