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Acute Knockdown of Kv4.1 Regulates Repetitive Firing Rates and Clock Gene Expression in the Suprachiasmatic Nucleus and Daily Rhythms in Locomotor Behavior
Reducing Kv4.1 Quickly Changes Nerve Cell Firing, Daily Clock Gene Activity, and Movement Rhythms
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Abstract
Knockdown of in mouse neurons significantly increased repetitive firing rates during the day (6.4 ± 0.5 Hz) and night (4.3 ± 0.6 Hz).
- Repetitive firing rates in Kv4.1-targeted neurons were notably higher compared to nontargeted neurons (day: 3.1 ± 0.5 Hz; night: 1.6 ± 0.3 Hz).
- The current (I) was significantly reduced in Kv4.1-targeted neurons (day: 80.3 ± 11.8 pA/pF; night: 55.3 ± 7.7 pA/pF) compared to nontargeted neurons (day: 121.7 ± 10.2 pA/pF; night: 120.6 ± 16.5 pA/pF).
- The effects of Kv4.1 knockdown on firing rates and current were more pronounced at night.
- Nighttime input resistance increased significantly to 2256 ± 166 MΩ in Kv4.1-targeted neurons, versus 1143 ± 93 MΩ in nontargeted neurons.
- Acute knockdown of Kv4.1 also shortened the circadian period of spontaneous electrical activity, clock gene expression, and locomotor activity by approximately 0.5 hours.
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Key numbers
6.4 ± 0.5 Hz
Increase in Repetitive Firing Rate
Mean firing rate in -targeted neurons during the day
2256 ± 166 MΩ
Input Resistance Increase
Nighttime input resistance in -targeted neurons
0.5 h
Circadian Period Shortening
Reduction in circadian period of electrical activity due to knockdown