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Adenine base editor engineering reduces editing of bystander cytosines
Improved adenine base editors reduce unintended changes to nearby cytosines
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Abstract
The D108Q mutation in the adenosine deaminase TadA7.10 results in tenfold reduced cytosine deamination activity.
- Adenine base editors (ABEs) convert specific A-to-G sequences in DNA but also cause unwanted cytosine changes.
- The D108Q mutation reduces cytosine editing in ABE7.10, ABE8e, and ABE8s.
- The combination of D108Q with another mutation, V106W, lowers off-target effects on RNA.
- A different mutation, P48R, increases cytosine editing while significantly decreasing the rate of adenine editing.
- These findings enhance the capability of base editors for specific TC-to-TT or TC-to-TG conversions.
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