Targeted disruption of KRAS p.G12V mutation in colorectal cancer cells using electroporated Cas9 protein complexes

Direct electroporation of Cas9-sgRNA RNPs targeting KRAS p.G12V was evaluated in SW480 colorectal cancer cells.

• KRAS mutations are linked to tumor growth and resistance to treatment in colorectal cancer.
• Efficient delivery of genome editing tools like Cas9 RNPs is crucial for effective therapeutic applications.
• Direct electroporation may enhance the uptake of Cas9 RNPs in targeted cells.
• The study assessed the efficiency of editing, potential unintended effects, and cell viability after treatment.

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