The ameliorative role of Aloe vera-loaded chitosan nanoparticles on Staphylococcus aureus induced acute lung injury: Targeting TLR/ NF-κB signaling pathways

Apr 18, 2024Open veterinary journal

Aloe vera nanoparticles reduce Staphylococcus aureus lung injury by affecting immune response pathways

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Regenerative Health on OpenScience ↗PubMed ↗DOI ↗

Abstract

The maximum entrapment efficiency of the chitosan nanoparticles was 95.5% ± 1.25%.

Chitosan nanoparticles loaded with Aloe vera exhibited an average particle size of approximately 60 nm.
The minimum inhibitory concentration and minimum bactericidal concentration were determined to be 5 and 10 µg/ml, respectively, indicating strong antibacterial activity against Staphylococcus aureus.
Infected rats showed increased mRNA expression of TLR2 and TLR4, suggesting heightened inflammatory responses.
Treatment with Aloe vera-loaded chitosan nanoparticles resulted in a reversal of the repression of TLR2 and TLR4 mRNA expression in treated rats compared to untreated ones.

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BACKGROUND: Acute lung injury (ALI) is a severe condition distinguished by inflammation and impaired gas exchange in the lungs.a common bacterium, can cause ALI through its virulence factors.is a medicinal plant that has been traditionally used to treat a variety of illnesses due to its anti-inflammatory properties. Chitosan nanoparticles are biocompatible and totally biodegradable materials that have shown potential in drug delivery systems. Staphylococcus aureus, Aloe vera

AIM: To explore the antibacterial activity of-loaded chitosan nanoparticles (AV-CS-NPs) againstandwith advanced techniques. Aloe vera S. aureus in vitro in vivo

METHODS: The antibacterial efficacy of AV-CS-NPs was evaluated through a broth microdilution assay. In addition, the impact of AV-CS-NPs oninduced ALI in rats was examined by analyzing the expression of genes linked to inflammation, oxidative stress, and apoptosis. Furthermore, rat lung tissue was scanned histologically. The rats were divided into three groups: control, ALI, and treatment with AV-CS-NPs. S. aureus-

RESULTS: The AV-CS-NPs that were prepared exhibited clustered semispherical and spherical forms, having an average particle size of approximately 60 nm. These nanoparticles displayed a diverse structure with an uneven distribution of particle sizes. The maximum entrapment efficiency of 95.5% ± 1.25% was achieved. The obtained findings revealed that The minimum inhibitory concentration and minimum bactericidal concentration values were determined to be 5 and 10 ug/ml, respectively, indicating the potent bactericidal effect of the NPs. Also,infected rats explored upregulation in the mRNA expression ofandcompared to healthy control groups. AV-CS-NP treatment reverses the case where there was repression in mRNA expression ofandcompared to-treated rats. S. aureus TLR2TLR4TLR2TLR4S. aureus

CONCLUSION: These NPs can serve as potential candidates for the development of alternative antimicrobial agents.

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The ameliorative role of Aloe vera-loaded chitosan nanoparticles on Staphylococcus aureus induced acute lung injury: Targeting TLR/ NF-κB signaling pathways

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