Creating stable Arabidopsis plants with four identical chromosome sets using CRISPR gene editing

CRISPR/Cas9 genome editing successfully generated homozygous nulliplex mutants in tetraploid Arabidopsis thaliana.

• Targeted mutagenesis using CRISPR/Cas9 can modify specific gene loci in polyploid plants.
• Generating knockout mutants in polyploid species has been challenging due to multiple gene copies.
• The study focused on the TTG1 gene to assess the efficiency of creating mutant lines.
• Results indicate that tetraploid (4x) backgrounds can yield nulliplex mutants effectively.
• This method may enhance the understanding of genome dosage effects in plants.

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