Cell Recycling Damage in Aging Bone Marrow Cells and Its Link to Piezo1 Levels During Osteoporosis Progression

Piezo1 expression was downregulated in bone tissues of patients with osteoporosis and ovariectomized mice.

• Aging and estrogen deficiency in osteoporosis lead to oxidative stress that impairs bone marrow mesenchymal stromal cell (BMSC) function.
• Increased senescence markers were observed in BMSCs from osteoporotic conditions.
• Oxidative stress was associated with reduced Piezo1 expression and a shift in BMSC differentiation towards fat cells instead of bone-forming cells.
• Impaired autophagic flux in senescent BMSCs was noted, which could be restored by activating autophagy with rapamycin.
• Rapamycin treatment enhanced Piezo1 expression, alleviated senescence, and improved bone mass and structure in ovariectomized mice.

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