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Advances in base and prime gene editing tools using the CRISPR/Cas system
Updated
Abstract
Base editors (BEs) and prime editors (PEs) are efficient gene editing technologies utilizing the CRISPR/Cas system.
- Cytosine base editors (CBEs) convert C to T, while adenine base editors (ABEs) convert A to G.
- Glycosylase base editors (GBEs) facilitate the conversion of C to G.
- Dual-base editors (DBEs) enable simultaneous conversion of C to T and A to G.
- These editing technologies have been applied effectively in various animals, plants, and microorganisms.
- BEs and PEs operate independently of DNA double-strand breaks or donor DNA, enhancing their utility.
- Current challenges for improving editing efficiency and precision are identified and discussed.
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