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Abstract
Plant base editing has evolved to include various systems, enhancing mutation accessibility and genomic targeting.
- The toolkit has expanded from basic cytosine and adenine editors to include dual-base and organelle-targeted systems.
- These advancements may facilitate the generation of beneficial alleles and modifications in gene function.
- Despite improvements, expanded base-editing capabilities do not guarantee predictable results in breeding.
- A comparison of editing efficiency, product purity, and specificity is provided, focusing on transversion editing.
- The review identifies technical limitations and bottlenecks faced in applying base editing from the lab to field settings.
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