Testing and improving a gene editing method in human stem cells turning into different cell types

Analysis of 1,996,260 sequenced cells reveals shared regulatory networks during cardiomyocyte differentiation.

• Perturb-seq effectively assesses the influence of genes and enhancers on development and disease pathways.
• Technical challenges have previously limited the use of Perturb-seq in stem-cell systems.
• Multiple CRISPRi modalities and sgRNA delivery systems were benchmarked across diverse genomic targets.
• The optimized protocol provides a cost-effective method to produce large-scale datasets.
• Results indicate connections between disease-associated enhancers and their regulatory roles in gene expression.

Simplified