Integrating Bioactive Graded Hydrogel with Radially Aligned Nanofibers to Dynamically Manipulate Wound Healing Process

Jul 11, 2024ACS applied materials & interfaces

Using a special gel combined with aligned fibers to actively control wound healing

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Regenerative Health on OpenScience ↗PubMed ↗DOI ↗

Abstract

The hybrid scaffold significantly accelerated wound healing and collagen deposition in a rat skin wound-healing model.

The scaffold incorporated gelatin methacryloyl hydrogels and electrospun poly(ε-caprolactone)/gelatin nanofibers.
Early-stage healing was enhanced by the release of a peptide mimicking vascular endothelial growth factor to promote blood vessel formation.
Later-stage healing involved a different peptide that encouraged cell movement and worked in conjunction with a specialized nanofiber layer.
The wound site exhibited a collagen ratio similar to that of normal skin, indicating effective tissue regeneration.
The scaffold's design allows for dynamic regulation of the wound healing process in stages.

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Skin wound healing is a complex process that requires appropriate treatment and management. Using a single scaffold to dynamically manipulate angiogenesis, cell migration and proliferation, and tissue reconstruction during skin wound healing is a great challenge. We developed a hybrid scaffold platform that integrates the spatiotemporal delivery of bioactive cues with topographical cues to dynamically manipulate the wound-healing process. The scaffold comprised gelatin methacryloyl hydrogels and electrospun poly(ε-caprolactone)/gelatin nanofibers. The hydrogels had graded cross-linking densities and were loaded with two different functional bioactive peptides. The nanofibers comprised a radially aligned nanofiber array layer and a layer of random fibers. During the early stages of wound healing, the KLTWQELYQLKYKGI peptide, which mimics vascular endothelial growth factor, was released from the inner layer of the hydrogel to accelerate angiogenesis. During the later stages of wound healing, the IKVAVS peptide, which promotes cell migration, synergized with the radially aligned nanofiber membrane to promote cell migration, while the nanofiber membrane also supported further cell proliferation. In an in vivo rat skin wound-healing model, the hybrid scaffold significantly accelerated wound healing and collagen deposition, and the ratio of type I to type III collagen at the wound site resembled that of normal skin. The prepared scaffold dynamically regulated the skin tissue regeneration process in stages to achieve rapid wound repair with clinical application potential, providing a strategy for skin wound repair.

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Integrating Bioactive Graded Hydrogel with Radially Aligned Nanofibers to Dynamically Manipulate Wound Healing Process

Abstract

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