Engineering artificial biosynthetic pathways for efficient microbial production of psilocybin and psilocin

Nov 7, 2025Metabolic engineering

Designed microbial systems for efficient production of psilocybin and psilocin

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Abstract

De novo biosynthesis of psilocybin achieved 557.91 mg/L in shake flasks and 2.00 g/L in a bioreactor.

  • Psychedelic-assisted therapy may be a promising option for treating depression.
  • Psilocybin, found in magic mushrooms, is recognized for its effectiveness against PTSD and treatment-resistant depression.
  • Current methods for extracting psilocybin are costly due to its low natural abundance.
  • Artificial biosynthetic pathways in Escherichia coli could improve psilocybin production by bypassing a key enzyme.
  • This approach may enhance the efficiency and commercial viability of psilocybin production.

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